If you’ve ordered a 10ml vial of peptide, you’ll see a small amount of white powder inside.

That’s the lyophilised peptide — a freeze-dried form of the compound.

Before you can use it for research, it needs to be mixed with Bacteriostatic Water to turn it into a clear liquid.

This process is called reconstitution.

Why Peptides Come as a Lyophilised (Freeze-Dried) Powder

Peptides are fragile and can break down quickly if left in water for long periods.

To make them last, manufacturers freeze-dry (lyophilise) them — removing all moisture while keeping the peptide stable.

That’s why it arrives as dry powder.

When you add sterile water later, it becomes active again and ready for research.

What You’ll Need

• 1 × 10ml peptide vial (white powder)

• 1 × vial of Bacteriostatic Water (0.9% benzyl alcohol)

• 1 × sterile syringe and needle

• 1 × alcohol wipe

• A clean, flat surface

Step-by-Step: Mixing Your Peptide

1. Clean your area
   Wash your hands and wipe down your workspace.
   Clean the tops of both vials (the peptide and the water) with an alcohol wipe.

2. Draw up the Bacteriostatic Water
   Use a sterile syringe to draw the amount of water you want to add.
   The more water you add, the weaker (less concentrated) your peptide solution will be — but it’s easier to measure.

3. Add the water slowly
   Insert the syringe into the peptide vial.
   Gently inject the water down the side of the vial, not directly onto the powder.
   This helps the powder dissolve evenly.

4. Let it dissolve
   Don’t shake the vial.
   Just gently roll or swirl it between your fingers until the powder disappears and the liquid is completely clear.

5. Store properly
   Keep the mixed peptide in the fridge (2°C to 8°C) — never freeze it once mixed.
   It stays good for 28 days if kept clean and cold.

Understanding Mixing Ratios (Easy Examples)

When you mix 10mg of peptide with water, you’re deciding how strong your liquid will be.

The “mg per ml” tells you how much peptide is in each millilitre (ml) of liquid.

Example 1: Strong Mix

Add 2ml of water to a 10mg peptide vial.
10mg ÷ 2ml = 5mg per 1ml

Results:

• 1ml = 5mg peptide

• 0.2ml (20 units) = 1mg peptide

Example 2: Medium Mix

Add 4ml of water to a 10mg peptide vial.
10mg ÷ 4ml = 2.5mg per 1ml

Results:

• 1ml = 2.5mg peptide

• 0.4ml (40 units) = 1mg peptide

Example 3: Easy-to-Calculate Mix

Add 10ml of water to a 10mg peptide vial.
10mg ÷ 10ml = 1mg per 1ml

Results:

• 1ml = 1mg peptide

• 0.5ml (50 units) = 0.5mg peptide

• 0.1ml (10 units) = 0.1mg peptide

Quick Reference (Peptide Strengths)

Peptide 10mg + 2ml water = 5mg per 1ml → 0.2ml = 1mg
Peptide 10mg + 4ml water = 2.5mg per 1ml → 0.4ml = 1mg
Peptide 10mg + 5ml water = 2mg per 1ml → 0.5ml = 1mg
Peptide 10mg + 10ml water = 1mg per 1ml → 1.0ml = 1mg

“5mg per 1ml” means every full 1ml in your syringe contains 5 milligrams of peptide.

Syringe Example

A 1ml insulin syringe is marked from 0 to 100 units:

• 100 units = 1.0ml

• 50 units = 0.5ml

• 20 units = 0.2ml

So if your mix is 1mg per 1ml, drawing 50 units (0.5ml) gives you 0.5mg of peptide.